Preparation, Characterization, and Chromatographic Evaluation of Nickel Oxide Deposited Silica Stationary Phase in Hydrophilic Interaction Liquid Chromatography

Nickel oxide deposited silica stationary phase was prepared by liquid deposition and characterized. Its chromatographic performance evaluated using several compounds such as typical polar benzimidazoles probes. The effects of mobile variables acetonitrile content, salt concentration, pH on the behavior, thermodynamics, kinetics these were investigated to reveal retention mechanism. results showed that exhibited a behavior hydrophilic interaction chromatography, multiple mechanisms including partitioning, adsorption, ion-exchange, electrostatic attraction, coordination interactions contributed solute retention. nickel electron-accepting sites electron-donating solutes resulted in mixed-mode phase, which could be very useful for enhancing selectivity analytes. batch-to-batch reproducibility acceptable with relative standard deviations probe less than 9.89%. successfully employed separation compounds, it better effect different from column commercial Zorbax NH2 column.